
NEXT GENERATION SEQUENCING NOVASEQ 6000
Nguyen Tuyet Mai2025-07-07T14:32:23+00:00
- Match data output, time to results, and cost per sample to study needs
- Configure sequencing method, flow cell type, and read length for a broad range of applications
- Increase lab efficiency with a simplified workflow and reduced hands-on time
- Load-and-go reagent cartridges represent an 80% reduction in consumables compared to the HiSeq Series of Systems
- Ready-to-use reagents require no preparation other than thawing and inversion, eliminating the need for user intervention, minimizing human error, and drastically reducing run setup time
- Radio-frequency identification (RFID)–encoded consumables enable automated reagent traceability and ensure compatibility of all sequencing reagents and flow cells
- Automated flow cell loading and onboard cluster generation minimize hands-on time
- Extended reagent shelf life supports efficient planning for future projects
- A 35-cycle kit increases workflow options, including COVIDSeq™ Test and counting applications, while lowering the cost per read
- Enhanced reagent chemistry streamlines the workflows increases the number of unique molecular identifiers (UMIs) to accommodate more complex indexing strategies, and supports the wide range of Illumina library preparation solutions
| Flow cell type |
SP |
S1 |
S2 |
S4 |
| Lanes per flow cell |
2 |
2 |
2 |
4 |
| Output per flow cell |
| 1 × 35 bp |
N/A |
N/A |
N/A |
280-350 Gb |
| 2 × 50 bp |
65-80 Gb |
134-167 Gb |
333-417 Gb |
N/A |
| 2 × 100 bp |
134-167 Gb |
266-333 Gb |
667-833 Gb |
1600- 2000 Gb |
| 2 × 150 bp |
200-250 Gb |
400-500 Gb |
1000- 1250 Gb |
2400- 3000 Gb |
| 2 × 250 bp |
325-400 Gb |
N/A |
N/A |
N/A |
| Single reads CPF |
0.65-0.8B |
1.3-1.6B |
3.3-4.1B |
8-10B |
| PE reads CPF |
1.3-1.6B |
2.6-3.2B |
6.6-8.2B |
16-20B |
| Quality scores |
| 1 × 35 bp |
Q30 ≥ 90% |
| 2 × 50 bp |
Q30 ≥ 90% |
| 2 × 100 bp |
Q30 ≥ 85% |
| 2 × 150 bp |
Q30 ≥ 85% |
| 2 × 250 bp |
Q30 ≥ 75% |
| Run time |
| 1 × 35 bp |
N/A |
N/A |
N/A |
~ 14 hr |
| 2 × 50 bp |
~ 13 hr |
~ 13 hr |
~ 16 hr |
N/A |
| 2 × 100 bp |
~ 19 hr |
~ 19 hr |
~ 25 hr |
~ 36 hr |
| 2 × 150 bp |
~ 25 hr |
~ 25 hr |
~ 36 hr |
~ 44 hr |
| 2 × 250 bp |
~ 38 hr |
N/A |
N/A |
N/A |