- Match data output, time to results, and cost per sample to study needs
- Configure sequencing method, flow cell type, and read length for a broad range of applications
- Increase lab efficiency with a simplified workflow and reduced hands-on time
- Load-and-go reagent cartridges represent an 80% reduction in consumables compared to the HiSeq Series of Systems
- Ready-to-use reagents require no preparation other than thawing and inversion, eliminating the need for user intervention, minimizing human error, and drastically reducing run setup time
- Radio-frequency identification (RFID)–encoded consumables enable automated reagent traceability and ensure compatibility of all sequencing reagents and flow cells
- Automated flow cell loading and onboard cluster generation minimize hands-on time
- Extended reagent shelf life supports efficient planning for future projects
- A 35-cycle kit increases workflow options, including COVIDSeq™ Test and counting applications, while lowering the cost per read
- Enhanced reagent chemistry streamlines the workflows increases the number of unique molecular identifiers (UMIs) to accommodate more complex indexing strategies, and supports the wide range of Illumina library preparation solutions
Flow cell type | SP | S1 | S2 | S4 |
Lanes per flow cell | 2 | 2 | 2 | 4 |
Output per flow cell | ||||
1 × 35 bp | N/A | N/A | N/A | 280-350 Gb |
2 × 50 bp | 65-80 Gb | 134-167 Gb | 333-417 Gb | N/A |
2 × 100 bp | 134-167 Gb | 266-333 Gb | 667-833 Gb | 1600- 2000 Gb |
2 × 150 bp | 200-250 Gb | 400-500 Gb | 1000- 1250 Gb | 2400- 3000 Gb |
2 × 250 bp | 325-400 Gb | N/A | N/A | N/A |
Single reads CPF | 0.65-0.8B | 1.3-1.6B | 3.3-4.1B | 8-10B |
PE reads CPF | 1.3-1.6B | 2.6-3.2B | 6.6-8.2B | 16-20B |
Quality scores | ||||
1 × 35 bp | Q30 ≥ 90% | |||
2 × 50 bp | Q30 ≥ 90% | |||
2 × 100 bp | Q30 ≥ 85% | |||
2 × 150 bp | Q30 ≥ 85% | |||
2 × 250 bp | Q30 ≥ 75% | |||
Run time | ||||
1 × 35 bp | N/A | N/A | N/A | ~ 14 hr |
2 × 50 bp | ~ 13 hr | ~ 13 hr | ~ 16 hr | N/A |
2 × 100 bp | ~ 19 hr | ~ 19 hr | ~ 25 hr | ~ 36 hr |
2 × 150 bp | ~ 25 hr | ~ 25 hr | ~ 36 hr | ~ 44 hr |
2 × 250 bp | ~ 38 hr | N/A | N/A | N/A |